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「Biophysics and Physicobiology」に Hiroyuki Iwamoto, Kazuhiro Oiwa, Kogiku Shiba, Kazuo Inaba による "X-ray diffraction recording from a small amount of fibrous protein materials oriented by a micro shear-flow cell" をJ-STAGEの早期公開版として掲載

2024年04月20日 学会誌

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が早期公開されました。

Hiroyuki Iwamoto, Kazuhiro Oiwa, Kogiku Shiba, Kazuo Inaba
"X-ray diffraction recording from a small amount of fibrous protein materials oriented by a micro shear-flow cell"

URL:https://doi.org/10.2142/biophysico.bppb-v21.0014


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Abstract
This paper describes a method for recording X-ray diffraction patterns from a small amount of fibrous protein materials while being oriented by using a micro shear-flow cell. This cell consists of two concentrically arranged glass tubes. The inner tube is stationary, while the outer one rotates at a high speed. The gap between the two tubes is about 100 μm, into which the suspension of fibrous protein materials is injected. By using synchrotron-radiation X-ray microbeams (diameter, 10 μm), clear diffraction images from oriented protein materials can be recorded. The required volume of the sample is only about 10 μl. This method can also be combined with the laser-flash photolysis of caged compounds. Examples of application of this method to the flagella of a green alga Chlamydomonas, and sperm of a tunicate Ciona are presented.

URL: https://doi.org/10.2142/biophysico.bppb-v21.0014



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