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「Biophysics and Physicobiology」に Kumari Sushmita, Sunita Sharma, Manish Singh Kaushik, Suneel Kateriya による "Algal rhodopsins encoding diverse signal sequence holds potential for expansion of organelle optogenetics" をJ-STAGEの早期公開版として掲載

2023年01月24日 学会誌

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が早期公開されました。

Kumari Sushmita, Sunita Sharma, Manish Singh Kaushik, Suneel Kateriya
"Algal rhodopsins encoding diverse signal sequence holds potential for expansion of organelle optogenetics"

URL:https://doi.org/10.2142/biophysico.bppb-v20.s008


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Abstract
Rhodopsins have been extensively employed for optogenetic regulation of bioelectrical activity of excitable cells and other cellular processes across biological systems. Various strategies have been adopted to attune the cellular processes at the desired subcellular compartment (plasma membrane, endoplasmic reticulum, Golgi, mitochondria, lysosome) within the cell. These strategies include- adding signal sequences, tethering peptides, specific interaction sites, or mRNA elements at different sites in the optogenetic proteins for plasma membrane integration and subcellular targeting. However, a single approach for organelle optogenetics was not suitable for the relevant optogenetic proteins and often led to the poor expression, mislocalization, or altered physical and functional properties. Therefore, the current study is focused on the native subcellular targeting machinery of algal rhodopsins. The N- and C-terminus signal prediction led to the identification of rhodopsins with diverse organelle targeting signal sequences for the nucleus, mitochondria, lysosome, endosome, vacuole, and cilia. Several identified channelrhodopsins and ion-pumping rhodopsins possess effector domains associated with DNA metabolism (repair, replication, and recombination) and gene regulation. The identified algal rhodopsins with diverse effector domains and encoded native subcellular targeting sequences hold immense potential to establish expanded organelle optogenetic regulation and associated cellular signaling.



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