「Biophysics and Physicobiology」にYoichi Nakatani et al. “Quantitative analyses of the equilibria among DNA complexes of a blue-light-regulated bZIP module, Photozipper”を掲載

2018 January 22 BPPB

日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が新規掲載されました。

Yoichi Nakatani and Osamu Hisatomi “Quantitative analyses of the equilibria among DNA complexes of a blue-light-regulated bZIP module, Photozipper”

【Significance】
Quantitative analyses of the equilibria in PZ–DNA-complex formation

Photozipper (PZ) is a light-regulated dimerizing module containing bZIP domain and a LOV domain of V. frigida aureochrome1. Here, we quantitatively investigated DNA-binding of PZ and proposed a quantitative model for the equilibria in PZ–DNA-complex formation. PZ exhibited about 10 times increase in its affinity for binding to the target sequence in a light-dependent manner. The blue-light-induced dimerization of LOV domains and coiled-coil formation by leucine zipper regions stabilize dimeric complex with the target DNA sequence. This work suggested that PZ could be a powerful module to light-control interactions with substrates for optogenetics.

Biophysics and Physicobiology, Vol.15, pp. 8-17

URL:https://www.jstage.jst.go.jp/article/biophysico/15/0/15_8/_article

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