「Biophysics and Physicobiology」に Kazuho Yoshida et al. “Chimeric microbial rhodopsins for optical activation of Gs-proteins”を掲載

2017 December 20 BPPB

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Kazuho Yoshida, Takahiro Yamashita, Kengo Sasaki, Keiichi Inoue, Yoshinori Shichida, Hideki Kandori “Chimeric microbial rhodopsins for optical activation of Gs-proteins”

Chimeric microbial rhodopsins for optical activation of Gs-proteins

Chimeric proteins of a light-driven proton pump GR containing the cytoplasmic loop of β2-adrenergic receptor (β2AR) activate Gs protein by light. In contrast, chimeric proteins of light-driven sodium pump IndiR2 or chloride pump NpHR containing the same loop of β2AR do not activate Gs protein at all. Light-induced difference FTIR spectroscopy observed largest helical structural perturbation for GR, which was further enhanced in the chimera. Similar structural dynamics that occur on the cytoplasmic side of GPCR are needed to design chimeric microbial rhodopsins.

Biophysics and Physicobiology, Vol.14, pp. 183-190


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