「Biophysics and Physicobiology」に 〈Special Issue: "Protein-Ligand Interactions"〉 Kentaro Ishii et al. “Mass spectrometric analysis of protein–ligand interactions”を掲載

2016 July 15 BPPB

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Kentaro Ishii, Masanori Noda, Susumu Uchiyama
“Mass spectrometric analysis of protein–ligand interactions”

Mass spectrometric analysis of protein–ligand interactions

Recent advancement in mass spectrometry (MS) enables us to know the molecular masses of protein ligand complexes without a disruption of non-covalent interactions. This method is called as MS under non-denaturing condition or native MS. Thus, one can know the stoichiometry of a protein–ligand interaction unambiguously. Also attempts to determine the dissociation constant of a protein–ligand interaction have been made by MS under a few assumptions. The location of the interaction and conformational change in a protein can be analyzed by hydrogen/deuterium exchange MS. This article describes how MS is effective in the study on protein–ligand interactions.

Biophysics and Physicobiology, Vol.13, pp. 87-95


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