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Pseudomonas aeruginosa PAO1 twitching on agar

Species name: Pseudomonas aeruginosa

Department of Biochemistry and Biomedical Sciences, McMaster University Lori L. Burrows

Pseudomonas aeruginosa is an antibiotic resistant bacterium that causes hospital-related infections, especially in people with bad burns, in intensive care units, and people with the disease cystic fibrosis. This movie shows how it can quickly spread across surfaces in a matter of minutes. This movie consists of 150 frames captured over a 10 minute window (15 frames per minute). The movie was then recorded at 5 frames per second to show the motility of the bacteria. They are using their type IV pili to move along the surface between the agar and the glass coverslip, via twitching motility. Due to technical issues, the focus was maintained manually over the 10 min timeframe. Video made by Lori L. Burrows, PhD and Hosam Khalil, MSc in the McMaster Biophotonics Facility, McMaster University.

(2015.06.22)

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