National Institute of Advanced Industrial Science and Technology (AIST) and Kanazawa University Kien Xuan Ngo, Noriyuki Kodera Taro Q.P. Uyeda
The first three quarter of this video were taken in F buffer containing 1 mM ATP and 1 µM cofilin, and the last one quarter was taken in the presence of 650 nM cofilin. In the presence of 1 µM cofilin, filaments were fully decorated along the length, and no severing was observed. In the presence of 650 nM cofilin, there were some bare zones, and severing (red arrowheads) occurred near the boundary of the bare zone and the cofilin clusters (white arrowheads), regardless of the size of cofilin clusters. Images were taken at 2 frames/s and played at 5 frames/s. The width of the imaged field: 320 nm, Z-scale: 0–12 nm.
(2015.06.18)