Department of Cell Biology, Duke University Medical Center Masaki Osawa
In order to reconstitute the Z ring (bacterial division machinery) in liposomes, artificial membrane targeted FtsZ (FtsZ-YFP-mts) was created by adding an amphipathic helix (Membrane targeting sequence: mts) to the C terminal end of FtsZ fused with YFP. In this movie, the purified FtsZ-YFP-mts with GTP forms large Z rings and generates constriction force in the tubular-shaped multilamellar liposomes without any other division related proteins. These Z rings behave quite similar to the one in vivo in the movie “Z ring formation and constriction 2”. References 1. Reconstitution of contractile FtsZ rings in liposomes. Osawa M, Anderson DE, Erickson HP. Science. 2008 May 9;320(5877):792-4. 2. Inside-out Z rings--constriction with and without GTP hydrolysis. Osawa M, Erickson HP. Mol Microbiol. 2011 Jul;81(2):571-9. 3. Chapter 1 - Tubular liposomes with variable permeability for reconstitution of FtsZ rings. Osawa M, Erickson HP. Methods Enzymol. 2009;464:3-17.
(2014.12.13)