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Non-centrosomal microtubules (MTs) traced by EB1-GFP in steady state cells

Department of Molecular Genetics, VIB and University of Antwerp, Antwerpen, Belgium, Professor Sophie Janssens

CHO HSPB1− (top) and CHO HSPB1+ (bottom) were transfected with EB1-GFP and imaged with spinning disk confocal microscopy. Image z-stacks comprising the centrosomal and Golgi region were acquired every 3 sec. The images on the left show the maximum intensity projections of all slices in the z-stack with inverted grey scale. Images on the right show these as cumulative maximum intensity projections over the elapsed time. Note the large number of non-centrosomal MTs (Microtubules) formed in CHO-HSPB1+ cells.

Plos One

(2014.10.25)

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