Department of Molecular Genetics, VIB and University of Antwerp, Antwerpen, Belgium, Professor Sophie Janssens
CHO HSPB1− (top) and CHO HSPB1+ (bottom) were transfected with EB1-GFP, treated with nocodazole and washed while imaging with spinning disk confocal microscopy. Image z-stacks comprising the complete volume of the cells were acquired every 5 sec. The movie shows the maximum intensity projections of all slices in the z-stack with inverted grey scale. Images on the right are shown with overlaid EB1-GFP tracks. Note much more non-centrosomal and centrosomal MTs were formed in HSPB1+.
(2014.10.25)