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Live cell microscopy of Vaccinia Virus (VACV) A36YdF/B5-YFP that appears in one frame to colocalise with F-actin

School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia Professor Timothy P. Newsome

HeLa cells were infected with A36YdF/B5-YFP (green), transfected with pE/L Lifeact-mRFP (red) and imaged for 80 seconds at 6–9 hours post infection. Colocalisation between a virus particle and F-actin is observed transiently at the beginning of the movie but is not associated with virus motility.

Plos Pathogen

(2014.10.08)

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