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Live cell microscopy of Vaccinia Vrus (VACV) A36Y112F/B5-YFP-induced actin nucleation

School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia Professor Timothy P. Newsome

HeLa cells were infected with A36Y112F/B5-YFP (green), transfected with pE/L Lifeact-mRFP (red) and imaged for 80 seconds at 6–9 hours post infection. A virus particle is seen undergoing rapid, presumably microtubule-dependent motility, before pausing and nucleating F-actin.

Plos Pathogen

(2014.10.08)

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