Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, UnitedStates of America Professor Debra L. Milton
Rainbow trout skin epithelial cells were isolated and incubated with 1 mM mannose prior to infection with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video suggests that the epithelial cells utilize a mannose-binding receptor to bind sugar residues on the surface of V. anguillarum strains lacking the O-antigen. The O-antigen may be predicted to mask these sugar residues on the bacterial surface blocking phagocytosis.
(2014.09.19)