Department of Molecular Biology, Umea° Centre for Microbial Research, Umea° University, Umea°, Sweden, and Southern Research Institute, Birmingham, Alabama, UnitedStates of America Professor Debra L. Milton
This video shows the ability of completed mutant to evade phagocytosis by the rainbow trout skin epithelial cells. Skin epithelial cells were isolated from rainbow trout and infected with 105 bacteria ml−1. Following infection, phagocytic activities of motile epithelial cells were tracked using live-cell microscopy. This video is representative of epithelial cell interactions also associated with the Δwzm and ΔwbhA mutants carrying the respective complementing wild-type gene. All three complemented mutant strains regained the ability to evade phagocytosis.
(2014.09.19)