Institute of Microbiology, D-BIOL, ETH Zu¨ rich, Zurich, Switzerland Professor Wolf-Dietrich Hardt
HeLa cells were infected with a 1:1 mixture of S.TmΔ4(pGFP) and S.TmSopE(pM2112, constitutive rfp-plasmid) at an m.o.i. of 5,6 minutes before movie acquisition. A 2.5 minute DIC movie was acquired using a 63× objective and 2-fold optovar, at 23 frames per second and is shown in real time. Images in the fluorescence channel showed that both S.TmΔ4 (non-invasive mutant) and S.TmSopE (invasive mutant) were associated with the membrane ruffle.
(2014.09.18)