Department of Chemistry and Biochemistry at UCSD Professor McCammon
This polymerization of actin both within the cell and in vitro has been studied experimentally for many years. One of the most interesting facts about actin polymerization is that the two ends of the filament do not grow at the same rate, but the barbed polymerizes 5-20 times faster than the pointed end. The basis for this asymmetry has never truly been understood. With advances in computational power in the past years, it has become possible to simulate the interaction of larger biomolecules, the polymerization of actin being one such system. Using Brownian dynamics simulations, the binding of a monomer to the end of a filament was simulated over a range of ionic strengths. The animation depicts two `fictional' trajectories intended to illustrate the basic concepts behind the simulations. For each trajectory, the monomer was started with a random orientation on a sphere surrounding the filament. By keeping track of the number of successful binding events at each end of the filament, a rate constant for binding can be calculated. This study showed that electrostatic interactions in fact lead to an asymmetry in polymerization rates between the two ends.
(2014.09.17)