Department of Physics and Bio-AFM Frontier Research Center, Kanazawa University Toshio Ando, Takayuki Uchihashi, and Noriyuki Kodera
The cantilever is oscillated in the z-direction at its resonant frequency. The position of the laser beam reflected back from the cantilever is monitored to measure the cantilever deflection. The oscillation amplitude reduced by tip tapping on the sample surface is maintained constant by moving the sample stage in the z-direction through feedback control. The resulting sample stage movement traces the sample surface. Therefore, the sample surface topography can be reconstructed by the use of a signal by which the z-scanner is driven.
Annual Review of Biophysics, 42: 393-414 (2013)
(2014.08.29)