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Simultaneous observation of Actin, Cofilin, Myosin by fluorescence microscopy

National Institute of Advanced Industrial Science and Technology Taro Uyeda

this video shows NBT-II cell (bladder cancer cell from mouse) co-expressing CFP-actin (top left), GFP-Cofilin (bottom left) and mRFP-myosin (top right). Reproduction in 600times (play speed: 10 frames/second, recording every 1 minute for 1 hour) In native NBT-II cell, the motor protein, actin has a high motility. However, the motility is decreased due to an over-expression of cofilin. Furthermore, we understand that both cofilin and myosin are not co-localized simultaneously

(2012.09.19)

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