Species name： Myxococcus xhantus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot
Myxococcus synthes spore coat by a group of gene (exoA–I). The function of this genes is encoding a capsular polysaccharide, and Wza-type export apparatus. The movie is showing the localization of structural components of the export apparatus. As an exception of proteobacteria, in Myxococcus the Wzc transmembrane domain and the kinase Wzc domain are carried by two distinct polypeptides named ExoC and ExoD/BtkA, respectively. BtkA is essential for Myxococcus sporulation, suggesting that BtkA can be used to localize Exo export sites. Z-sections of 4-h-old spores and 3D reconstructions further revealed that BtkA-sfGFP foci form at discrete sites around the spore periphery. The Z-stack recording was obtained on a 4-h-old sporulating cell.
(2014.08.27)