Species name： Myxococcus xanthus
Laboratoire de Chimie Bacte´rienne, CNRS UMR 7283, Aix-Marseille Universite´ , Institut de Microbiologie de la Me´diterrane´ e, Marseille, France Professor Tam Mignot
Myxococcus cells couldn’t complete the sporulation process when they spotted directly on an agar pad. To solve this problem under the live microscopy and elucidate how the Agl-Nfs machinery drives spore coat assembly, a microfluidic chamber assay has been developed. In this way, cells are immobilized and sporulate in liquid directly on the microscope stage. Under these conditions, viable spores were obtained approximately 250–300 min after glycerol addition.
(2014.08.27)