Hamamatsu University School of Medicine Shintaro Seto
Mycobacterium tuberculosis is an intracellular bacterium that can survive within phagocytosed macrophages. This ability relies on that mycobacteria inhibit the fusion of their phagosomes with lysosomes, phagolysosome biogenesis. Rab7 is one of small GTPases involved in phagolysosome biogenesis. This video demonstrates the localization of Rab7 to mycobacterial phagosomes from 30 min to 150 min postinfections (p.i.) by confocal lasar scanning microscopy. At 30 min p.i., GFP-fused Rab7 molecules (green) localize to the mycobacterial phagosome (red). However, Rab7 is dissociated from the phagosome at 150 min p.i. It is thought that mycobacteria inhibit phagolysosome biogenesis by the dissociation of Rab7 from their phagosomes.
Rab GTPases Regulating Phagosome Maturation Are Differentially Recruited to Mycobacterial Phagosomes (Traffic, 12 (4): 407-420, 2011)
Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome (BBRC, 387 (2), 272-277, 2009)
(2014.01.17)