National Institute of Advanced Industrial Science and Technology (AIST) and Kanazawa University Ngo Xuan Kien, 古寺哲幸、上田太郎
Individual cofilin molecules are too small to visualize by AFM. Thus, we fused the rod domain of α-actinin to the C-terminus of cofilin (cofilin-rod), so that transient binding of cofilin-rod to and dissociation from an actin filament was visualized by the presence of a rod-like structure sticking out of the actin filament (blue arrowhead). Note that no severing was observed near singly bound cofilin-rod molecules. Conditions: F buffer containing 1 mM ATP and 75 nM cofilin-rod, imaging rate: 4 frames/s, and playing rate: 5 frames/s. The width of the imaged field: 150 nm, Z-scale: 0–12 nm.
(2015.06.18)