「Biophysics and Physicobiology」に Takamitsu J Morikawa, Masayoshi Nishiyama, Keiko Yoshizawa, Hideaki Fujita, Tomonobu M Watanabe による "Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment" を掲載

2021年06月18日 学会誌

日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が公開されました。

Takamitsu J Morikawa, Masayoshi Nishiyama, Keiko Yoshizawa, Hideaki Fujita, Tomonobu M Watanabe
"Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment"

【Significance】
This study describes the effect of the environment surrounding the chromophore of GFP variants on their spectrum. A small structural distortion by few glycine residue insertions brings water molecule cavities significantly closer to the chromophore, altering the fluorescence dependence on pH, temperature, pressure, and solution hydrophobicity. We investigated these dependencies in various chromophores. While amino acid substitution strongly alters fluorescence through the different amino acid characteristics, glycine insertion can slightly alter the arrangement of amino acids in a β-strand. The present data provide important information regarding the protein structure and the formation of a hydrogen bond network with the chromophore.

Biophysics Physicobiology, 2021, Volume 18, Pages 145-158



URL:https://doi.org/10.2142/biophysico.bppb-v18.016

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