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「Biophysics and Physicobiology」にShuya Ishii et al. “Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied with in vitro motility assays”を掲載

2019年02月04日 学会から

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Shuya Ishii, Madoka Suzuki, Shin’ichi Ishiwata, Masataka Kawai “Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied with in vitro motility assays”

【Significance】
Tropomyosin's HCM mutants modulate in vitro sliding mechanism of actomyosin

In vitro motility assay was used to examine regulatory functions of thin filaments that contain tropomyosin mutants V95A or D175N, which are known to cause hypertrophic cardiomyopathy (HCM) in humans. The sliding velocity during relaxation was significantly increased in these mutants compared to wild type (WT) tropomyosin. Ca2+-activatable sliding force decreased in V95A and increased in D175N. The duty ratio determined at the steady state of force generation at saturating [Ca2+] decreased in V95A and increased in D175N. We conclude that HCM mutants of tropomyosin differently modulate molecular processes of the actomyosin interaction.


Biophysics and Physicobiology, Vol.16, pp. 28-40
URL:https://doi.org/10.2142/biophysico.16.0_28


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