「Biophysics and Physicobiology」にAkira Kitamura et al. “Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy ”を掲載

2018年01月19日 学会誌

日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が新規掲載されました。

Akira Kitamura and Masataka Kinjo “Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy”

【Significance】
A straight forward procedure to determine appropriate diffusion coefficient in living cells using FRAP


Fluorescence recovery after photobleaching (FRAP), that is widely used for analysis of molecular dynamics, allows quantitative determination of static and dynamic properties of molecules of interest. To know diffusion states of rapidly-diffusing fluorescent molecules such as green fluorescent protein (GFP) monomers in living cells, we proposed a straightforward procedure for measuring diffusion coefficients using FRAP with an epi-fluorescence microscope. In this procedure, the apparent spreading of the effective radius of the photobleached area works as a correction factor. This procedure can be used to measure the diffusion coefficients of almost any fluorescently-labeled fusion protein in living cells without using any specialized microscopy.

Biophysics and Physicobiology, Vol.15, pp. 1-7

URL:https://www.jstage.jst.go.jp/article/biophysico/15/0/15_1/_article

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