2017年02月13日 掲載 (Published 02/13/2017)
日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が新規掲載されました。
Kazuki Takahashi, Masato Sunohara, Takuya Terai, Shigefumi Kumachi and Naoto Nemoto “Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions”
Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display
mRNA display is a robust technology to identify functional proteins from large libraries by in vitro selection, which involves covalently fusing the mRNA to its coding protein via a puromycin-linker. However, low fusion efficiency for some proteins, in particular antibodies, remained to be solved. Here, we describe improvement of the mRNA-protein fusion efficiency of a single-domain antibody by an electrophoresis mobility shift assay-based selection of mRNA display molecules. We believe the work makes the in vitro selection of affinity reagents using mRNA display more user friendly and practical.
Biophysics and Physicobiology, Vol.14, pp. 23-28