Title
Advanced Insight into Protein Motions - in silico, in vitro and in vivo Studies

Organizers
Daizo Hamada (Kobe University), George Chikenji (Nagoya University)

Synopsis
Numerous biological phenomena even in disease state are associated with structures and motions of proteins. Recent studies clarified importance of aggregation phenomena even under normal conditions, which are traditionally considered to be less important for biological systems. Recent developments in methodologies to analyse protein motions including a rapid or single molecular observation techniques provide detail view in each event with high spatial and time resolutions. Unlike a pure in vitro system, however, numerous molecules including proteins themselves dynamically interact with each other to regulate biological phenomena in vivo. Under such conditions, proteins potentially behave differently from those under in vitro conditions due to the effects of macromolecular crowding etc. In-cell NMR technique directly observes motions of target proteins in vivo which are generally less predictable from data based on in vitro studies. Several developments in in silico techniques are also being constracted to overcome these problems.
   In this symposium, we focus on the above mentioned novel methodologies. The technological advancements provide significant insights into the motions of proteins that can further clarify more realistic images of protein molecules under various environments including physiological conditions.

Speakers
・ Tetsunari Kimura (IMS): Experimental Investigation of Protein Folding Funnel
・ Nobuhiko Tokuriki (University of Cambridge): Structural and functional plasticity promote smooth evolutionary transition of enzyme
・ Masahiro Shirakawa (Kyoto University): Folding stability of proteins in mammalian cells investigated by in-cell NMR spectroscopy
・ Motomasa Tanaka, Yumiko Ohhashi (RIKEN): The oligomers of a yeast prion Sup35-NM determine prion strain phenotypes
・ Yuichi Harano (Osaka University): Theoretical studies for biomolecular behavior in a liquid state - from the view point of molecular crowding -
・ Yutaka Ito ( Tokyo Metropolican University): Seeing proteins inside living cells: strucutres and dynamics of proteins by in-cell NMR



Time table of this symposium was changed as follows;

Speaker Original Changed
Kimura 13:50 13:50
Tokuriki 14:12 14:05
Shirakawa 14:22 14:15
Tanaka 14:44 14:35
Harano 15:06 14:55
Ito 15:28 15:10

All the speakers and title of the talks were not changed.



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